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| − | <h3>And Yet Another Subheading</h3> | + | <h3>Chassis Organisms</h3> |
| − | <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Maecenas ac quam risus, at tempus | + | <p>We genetically modified <i>Escherichia coli</i> and Lactococcus lactis in our project. The genes were constructed in E. coli and transformed into <i>Lactococcus lactis</i> using a shuttle vector known as pTRKH2. Both organisms are level 1 (low risk) safety level organisms.</p> |
| − | justo. Sed dictum rutrum massa eu volutpat. Quisque vitae hendrerit sem. Pellentesque lorem felis,
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| − | ultricies a bibendum id, bibendum sit amet nisl. Mauris et lorem quam. Maecenas rutrum imperdiet
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| − | vulputate. Nulla quis nibh ipsum, sed egestas justo. Morbi ut ante mattis orci convallis tempor.
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| − | Etiam a lacus a lacus pharetra porttitor quis accumsan odio. Sed vel euismod nisi. Etiam convallis
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| − | rhoncus dui quis euismod. Maecenas lorem tellus, congue et condimentum ac, ullamcorper non sapien.
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| − | Donec sagittis massa et leo semper a scelerisque metus faucibus. Morbi congue mattis mi.
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| − | Phasellus sed nisl vitae risus tristique volutpat. Cras rutrum commodo luctus.</p>
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| − | <p>Phasellus odio risus, faucibus et viverra vitae, eleifend ac purus. Praesent mattis, enim | + | <h3>Safety Training</h3> |
| − | quis hendrerit porttitor, sapien tortor viverra magna, sit amet rhoncus nisl lacus nec arcu. | + | <p>Our safety training was performed by Utah State University Environmental Health and Safety Office and covered chemical hygiene principles, spill prevention, hazardous waste management, and fire safety. </p> |
| − | Suspendisse laoreet metus ut metus imperdiet interdum aliquam justo tincidunt. Mauris dolor urna, | + | <h3>Laws and Regulations that Govern Biosafety in Research Laboratories in Our Country</h3> |
| − | fringilla vel malesuada ac, dignissim eu mi. Praesent mollis massa ac nulla pretium pretium. | + | <p>Information concerning relevant laws and regulations in the United States of America may be found on the U.S. Department of Health and Human Services website at <a href="http://www.phe.gov/s3/legal/Pages/laws.aspx">http://www.phe.gov/s3/legal/Pages/laws.aspx.</a></p> |
| − | Maecenas tortor mauris, consectetur pellentesque dapibus eget, tincidunt vitae arcu. | + | |
| − | Vestibulum purus augue, tincidunt sit amet iaculis id, porta eu purus.</p> | + | <h3>Risks Posed at the Laboratory Stage and Actions Taken to Reduce Risks</h3> |
| | + | <pThe two main risks posed by our project are exposure to ethidium bromide and releasing genetically modified organisms into the environment. To reduce exposure or release of the genetically modified bacteria we wear rubber gloves and sterilize waste with bleach and/or autoclaving. Biological materials were handled in a biosafety cabinet/laminar flow hood and on open benches. </p> |
| | + | <pEthidium bromide is the main material that we work with that is known to be hazardous, and we wear nitrile rubber gloves when handling this chemical and use the same pipette to transfer it to agarose solutions inside of a chemical hood.</p> |
| | + | <h3>How Our Project Would Be Used in the Real World</h3> |
| | + | <pOur project would be used in a factory, in a consumer product that ordinary people buy, and in food (cheese cultures).</p> |
| | + | <h3>Future Risks If Our Project Was Fully Developed and Steps That Could Be Taken to Reduce Risks</h3> |
| | + | <p>There is a relatively low level of risk from the bacteria themselves, even though they would be used in food production. The primary concern that may arise is the fact that the bacteria have an antibiotic resistance (erythromycin) in the vector plasmid used to transform our construct into the bacteria. That antibiotic resistance could potentially spread to other bacteria. Since erythromycin is primarily used in treating acne, it's unlikely that the <i>Lactococcus lactis</i> used in cheese culture will come into contact with bacteria causing acne on human skin enough to spread the resistance. Further work that could be done to reduce risk further might be work with a cre/lox system in the plasmid to remove the antibiotic resistance altogether after the plasmid is known to be in the cells.</p> |
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| | </article> | | </article> |
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