Difference between revisions of "Team:Amsterdam//Project/Synthetic biology/Dependecies"

One can construct a synthetic consortium between two species with a variety of methods. Synechocystis could simply be engineered to export a carbon source (such as glucose) for E. coli in a glucose-absent medium. Although such a commensal relationship would definitely under certain conditions be considered stable, E. coli alone is the benefactor - there is no drive for Synechocystis to produce this carbon source. Increased stability is possible by creating a reciprocal relationship between the two species. Therefore, how can Cyanobacteria benefit or depend on E. coli’s presence? Cyanobacteria manufacturers it’s own food and has grown for centuries requiring only sunlight and water, in addition to inorganic elements. In fact, E. coli’s presence actually harms Cyanobacteria by limiting access to precious sunlight. Herein lies this module’s focus: Genetic engineering of an E. Coli dependent Synechocystis for the formation of stable synthetic consortia.