Difference between revisions of "Team:Pasteur Paris/Week 1"

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<div style="text-align: left">
 
<div style="text-align: left">
 
<ol>
 
<ol>
−
<li style="text-align:left">Resuspension of Biobrick (BB) DNA in 15µL of DNAse, RNAse free water: BBa_K808007,  BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000,  BBa_K316003.</li><br>
+
<li style="text-align:left">Resuspension of Biobrick (BB) DNA in 15 µl of DNAse, RNAse free water: BBa_K808007,  BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000,  BBa_K316003.</li><br>
 
<li style="text-align:left">Storage at -20°C.</li><br>
 
<li style="text-align:left">Storage at -20°C.</li><br>
−
<li style="text-align:left">Transformation in DH5α Competent cells  purchased from Invitrogen (# 18265-017) was done following the transformation protocol. (BBa_K808007, BBa_K808010, BBa_K808011,  BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003).</li><br>
+
<li style="text-align:left">Transformation in DH5α Competent cells  purchased from Invitrogen (# 18265-017) was done following the transformation protocol. (BBa_K808007, BBa_K808010, BBa_K808011,  BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003).</li><br>
−
<li style="text-align:left">Cells were plated on LB + chloramphenicol 34µg/mL (Cm34). </li><br>
+
<li style="text-align:left">Cells were plated on LB + chloramphenicol 34 µg/ml (Cm34). </li><br>
−
<li style="text-align:left">Incubation  overnight. </li><br>
+
<li style="text-align:left">Incubation  overnight at 37°C. </li><br>
 
<li style="text-align:left">Storage at 4°C. </li><br>
 
<li style="text-align:left">Storage at 4°C. </li><br>
 
<li style="text-align:left">Plasmid  purification following the MiniPrep protocol. </li><br>
 
<li style="text-align:left">Plasmid  purification following the MiniPrep protocol. </li><br>
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     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td width="173" valign="top"><p><strong>Clone 1</strong></p></td>
+
       <td width="173" valign="top"><p align="center"><strong>Clone 1</strong></p></td>
−
       <td width="173" valign="top"><p><strong>Clone 2</strong></p></td>
+
       <td width="173" valign="top"><p align="center"><strong>Clone 2</strong></p></td>
−
       <td width="173" valign="top"><p><strong>Clone 3</strong></p></td>
+
       <td width="173" valign="top"><p align="center"><strong>Clone 3</strong></p></td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td width="100" valign="top"><p><strong>808007</strong></p></td>
+
       <td width="100" valign="top"><p align="center"><strong>808007</strong></p></td>
−
       <td width="173" valign="top"><p>73.4</p></td>
+
       <td width="173" valign="top"><p align="center">73.4</p></td>
−
       <td width="173" valign="top"><p>44.3</p></td>
+
       <td width="173" valign="top"><p align="center">44.3</p></td>
−
       <td width="173" valign="top"><p>56.3</p></td>
+
       <td width="173" valign="top"><p align="center">56.3</p></td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td width="100" valign="top"><p><strong>808010</strong></p></td>
+
       <td width="100" valign="top"><p align="center"><strong>808010</strong></p></td>
−
       <td width="173" valign="top"><p>40.8</p></td>
+
       <td width="173" valign="top"><p align="center">40.8</p></td>
−
       <td width="173" valign="top"><p>27.5</p></td>
+
       <td width="173" valign="top"><p align="center">27.5</p></td>
−
       <td width="173" valign="top"><p>29.5</p></td>
+
       <td width="173" valign="top"><p align="center">29.5</p></td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td width="100" valign="top"><p><strong>808011</strong></p></td>
+
       <td width="100" valign="top"><p align="center"><strong>808011</strong></p></td>
−
       <td width="173" valign="top"><p>28.8</p></td>
+
       <td width="173" valign="top"><p align="center">28.8</p></td>
−
       <td width="173" valign="top"><p>30.4</p></td>
+
       <td width="173" valign="top"><p align="center">30.4</p></td>
−
       <td width="173" valign="top"><p>18.5</p></td>
+
       <td width="173" valign="top"><p align="center">18.5</p></td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td width="100" valign="top"><p><strong>808012</strong></p></td>
+
       <td width="100" valign="top"><p align="center"><strong>808012</strong></p></td>
−
       <td width="173" valign="top"><p>49.5</p></td>
+
       <td width="173" valign="top"><p align="center">49.5</p></td>
−
       <td width="173" valign="top"><p> N/A </p></td>
+
       <td width="173" valign="top"><p align="center"> N/A </p></td>
−
       <td width="173" valign="top"><p>56.5</p></td>
+
       <td width="173" valign="top"><p align="center">56.5</p></td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td width="100" valign="top"><p><strong>808013</strong></p></td>
+
       <td width="100" valign="top"><p align="center"><strong>808013</strong></p></td>
−
       <td width="173" valign="top"><p>18.8</p></td>
+
       <td width="173" valign="top"><p align="center">18.8</p></td>
−
       <td width="173" valign="top"><p>26</p></td>
+
       <td width="173" valign="top"><p align="center">26</p></td>
−
       <td width="173" valign="top"><p>15.7</p></td>
+
       <td width="173" valign="top"><p align="center">15.7</p></td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td width="100" valign="top"><p><strong>808014</strong></p></td>
+
       <td width="100" valign="top"><p align="center"><strong>808014</strong></p></td>
−
       <td width="173" valign="top"><p>30</p></td>
+
       <td width="173" valign="top"><p align="center">30</p></td>
−
       <td width="173" valign="top"><p>41.4</p></td>
+
       <td width="173" valign="top"><p align="center">41.4</p></td>
−
       <td width="173" valign="top"><p>37.4</p></td>
+
       <td width="173" valign="top"><p align="center">37.4</p></td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td width="100" valign="top"><p><strong>316003</strong></p></td>
+
       <td width="100" valign="top"><p align="center"><strong>316003</strong></p></td>
−
       <td width="173" valign="top"><p>51.5</p></td>
+
       <td width="173" valign="top"><p align="center">51.5</p></td>
−
       <td width="173" valign="top"><p>23.5</p></td>
+
       <td width="173" valign="top"><p align="center">23.5</p></td>
−
       <td width="173" valign="top"><p>36.1</p></td>
+
       <td width="173" valign="top"><p align="center">36.1</p></td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td width="100" valign="top"><p><strong>936000</strong></p></td>
+
       <td width="100" valign="top"><p align="center"><strong>936000</strong></p></td>
−
       <td width="173" valign="top"><p>15.2</p></td>
+
       <td width="173" valign="top"><p align="center">15.2</p></td>
−
       <td width="173" valign="top"><p>17.7</p></td>
+
       <td width="173" valign="top"><p align="center">17.7</p></td>
−
       <td width="173" valign="top"><p>13.8</p></td>
+
       <td width="173" valign="top"><p align="center">13.8</p></td>
 
     </tr>
 
     </tr>
 
   </table>
 
   </table>

Revision as of 19:29, 17 September 2015

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 1

06/01 - 06/05




  1. Resuspension of Biobrick (BB) DNA in 15 µl of DNAse, RNAse free water: BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003.

  2. Storage at -20°C.

  3. Transformation in DH5α Competent cells purchased from Invitrogen (# 18265-017) was done following the transformation protocol. (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003).

  4. Cells were plated on LB + chloramphenicol 34 µg/ml (Cm34).

  5. Incubation overnight at 37°C.

  6. Storage at 4°C.

  7. Plasmid purification following the MiniPrep protocol.

BBa

Concentration (ng/µL)

Clone 1

Clone 2

Clone 3

808007

73.4

44.3

56.3

808010

40.8

27.5

29.5

808011

28.8

30.4

18.5

808012

49.5

N/A

56.5

808013

18.8

26

15.7

808014

30

41.4

37.4

316003

51.5

23.5

36.1

936000

15.2

17.7

13.8

 



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