Difference between revisions of "Team:Pasteur Paris/Week 4"
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<table border="1" cellspacing="0" cellpadding="0" width="382"> | <table border="1" cellspacing="0" cellpadding="0" width="382"> | ||
<tr> | <tr> | ||
− | <td width="197" valign=" | + | <td width="197" valign="center"><p align="center">BBa_K808026 </p></td> |
<td width="60" valign="top"><p align="center">Clone 1 </p></td> | <td width="60" valign="top"><p align="center">Clone 1 </p></td> | ||
<td width="64" valign="top"><p align="center">Clone 2 </p></td> | <td width="64" valign="top"><p align="center">Clone 2 </p></td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="197" valign="top"><p align="center">DNA ( | + | <td width="197" valign="top"><p align="center">DNA (250 ng) Volume </p></td> |
<td width="60" valign="top"><p align="center">11.6 </p></td> | <td width="60" valign="top"><p align="center">11.6 </p></td> | ||
<td width="64" valign="top"><p align="center">7.4 </p></td> | <td width="64" valign="top"><p align="center">7.4 </p></td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="197" valign="top"><p align="center"> | + | <td width="197" valign="top"><p align="center">Buffer 10X Volume </p></td> |
<td width="60" valign="top"><p align="center">5.0 </p></td> | <td width="60" valign="top"><p align="center">5.0 </p></td> | ||
<td width="64" valign="top"><p align="center">5.0 </p></td> | <td width="64" valign="top"><p align="center">5.0 </p></td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="219" valign="top"><p align="center">Concentration (ng/ | + | <td width="219" valign="top"><p align="center">Concentration (ng/µl)</p></td> |
<td width="76" valign="top"><p align="center">21.5</p></td> | <td width="76" valign="top"><p align="center">21.5</p></td> | ||
<td width="79" valign="top"><p align="center">33.8</p></td> | <td width="79" valign="top"><p align="center">33.8</p></td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="219" valign="top"><p align="center">OD(260 nm)</p></td> | + | <td width="219" valign="top"><p align="center">OD (260 nm)</p></td> |
− | <td width="76" valign="top"><p align="center">0. | + | <td width="76" valign="top"><p align="center">0.43</p></td> |
− | <td width="79" valign="top"><p align="center">0. | + | <td width="79" valign="top"><p align="center">0.67</p></td> |
− | <td width="80" valign="top"><p align="center">0. | + | <td width="80" valign="top"><p align="center">0.53</p></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="219" valign="top"><p align="center">OD(260 nm)/OD (280nm)</p></td> | + | <td width="219" valign="top"><p align="center">OD (260 nm) / OD (280nm)</p></td> |
<td width="76" valign="top"><p align="center">1.69</p></td> | <td width="76" valign="top"><p align="center">1.69</p></td> | ||
<td width="79" valign="top"><p align="center">1.81</p></td> | <td width="79" valign="top"><p align="center">1.81</p></td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="219" valign="top"><p align="center">OD(260 nm)/OD ( | + | <td width="219" valign="top"><p align="center">OD (260 nm) / OD (230 nm)</p></td> |
<td width="76" valign="top"><p align="center">2.64</p></td> | <td width="76" valign="top"><p align="center">2.64</p></td> | ||
<td width="79" valign="top"><p align="center">1.56</p></td> | <td width="79" valign="top"><p align="center">1.56</p></td> | ||
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<br> | <br> | ||
<p align="left">- Gel migration of the BioBrick K808026 on a 0,8% Agarose Gel (1h at 130V):</p> <img src="https://static.igem.org/mediawiki/2015/4/4a/IGEM_Pasteur_gel_3_week_4.png" alt="l" width="449" height="336" /><br clear="all" /> | <p align="left">- Gel migration of the BioBrick K808026 on a 0,8% Agarose Gel (1h at 130V):</p> <img src="https://static.igem.org/mediawiki/2015/4/4a/IGEM_Pasteur_gel_3_week_4.png" alt="l" width="449" height="336" /><br clear="all" /> | ||
− | <strong><u>Figure 6</u></strong><u>:</u> Results of the | + | <strong><u>Figure 6</u></strong><u>:</u> Results of the gel migration (BBa_808026). </p><br> |
<li style="text-align:left">Bacterial culture of BBa_K1095000 on a Petri dish LB+Cm34. </li><br> | <li style="text-align:left">Bacterial culture of BBa_K1095000 on a Petri dish LB+Cm34. </li><br> | ||
<li style="text-align:left">Plasmid purification of BBa_808026 following the MidiPrep and MiniPrep protocol. </li> | <li style="text-align:left">Plasmid purification of BBa_808026 following the MidiPrep and MiniPrep protocol. </li> | ||
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<table border="1" cellspacing="0" cellpadding="0" width="382"> | <table border="1" cellspacing="0" cellpadding="0" width="382"> | ||
<tr> | <tr> | ||
− | <td width="197" valign=" | + | <td width="197" valign="center"><p align="center">BBa_K808026 </p></td> |
<td width="60" valign="top"><p align="center">Clone 1 </p></td> | <td width="60" valign="top"><p align="center">Clone 1 </p></td> | ||
<td width="64" valign="top"><p align="center">Clone 2 </p></td> | <td width="64" valign="top"><p align="center">Clone 2 </p></td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="197" valign="top"><p align="center">[DNA] C (ng/ | + | <td width="197" valign="top"><p align="center">[DNA] C (ng/µl) </p></td> |
<td width="60" valign="top"><p align="center">21.5 </p></td> | <td width="60" valign="top"><p align="center">21.5 </p></td> | ||
<td width="64" valign="top"><p align="center">33.8 </p></td> | <td width="64" valign="top"><p align="center">33.8 </p></td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="197" valign="top"><p align="center">DNA ( | + | <td width="197" valign="top"><p align="center">DNA (250 ng) Volume </p></td> |
<td width="60" valign="top"><p align="center">11.6 </p></td> | <td width="60" valign="top"><p align="center">11.6 </p></td> | ||
<td width="64" valign="top"><p align="center">7.4 </p></td> | <td width="64" valign="top"><p align="center">7.4 </p></td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="197" valign="top"><p align="center"> | + | <td width="197" valign="top"><p align="center">Buffer 10X Volume </p></td> |
<td width="60" valign="top"><p align="center">5.0 </p></td> | <td width="60" valign="top"><p align="center">5.0 </p></td> | ||
<td width="64" valign="top"><p align="center">5.0 </p></td> | <td width="64" valign="top"><p align="center">5.0 </p></td> |
Revision as of 19:46, 17 September 2015
Week 1Week 2Week 3Week 4Week 5Week 6Week 7Week 8Week 9Week 10Week 11Week 12Week 13Week 14Week 15 |
Week 4
Figure 5: DNA Extraction by using the QiAprep spin protocol for a bioassay and digest. - OD measurement using a Spectrophotometer:
- Gel migration of the BioBrick K808026 on a 0,8% Agarose Gel (1h at 130V): Figure 6: Results of the gel migration (BBa_808026).
Figure 7: DNA Extraction by using the QiAprep spin protocol for a bioassay and digest. |