Difference between revisions of "Team:Pasteur Paris/Week 10"
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Complete with water to 1l</p> | Complete with water to 1l</p> | ||
<p style="text-align:left">When this flask was autoclaved some ingredients were added in aseptical conditions:</p> | <p style="text-align:left">When this flask was autoclaved some ingredients were added in aseptical conditions:</p> | ||
− | <p style="text-align:left"> | + | <p style="text-align:left">1 ml of 1M Magnesium sulphate MgSO4 (6.02 g in 50 ml H2O)<br /> |
1 ml of 0.1 M of Calcium Chloride CaCl2 (0.55 g in 50 ml of H2O)</p> | 1 ml of 0.1 M of Calcium Chloride CaCl2 (0.55 g in 50 ml of H2O)</p> | ||
<p style="text-align:left">A mistake was done yesterday but this time an other did too: The last ingredients were added but they were not sterile so the medium failed again.</p> | <p style="text-align:left">A mistake was done yesterday but this time an other did too: The last ingredients were added but they were not sterile so the medium failed again.</p> |
Revision as of 21:37, 17 September 2015
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Week 10 Toxicity and solubility of TPA (Terephthalic acid)
Preparation of M9 and minimal media Solution 2: (agar) Solution 3: (sugar)
When the three parts have been autoclaved, 2 more ingredients must be added to the medium aseptically: At the end the volume of Agar was exceeded (350 ml and not 333 ml). So we had to redo this experience the next day. 05-08-15 – Experiment: Preparation of M9 medium – VB, PV Aim: Get culture medium for BAP1 Pfeifer cells. Protocol: (for 1 l medium) Agar was directly put in a bottle and salts were dissolved in a beaker with water. 6 g of Sodium Phosphate Na2HPO4.H2O When this flask was autoclaved some ingredients were added in aseptical conditions: 1 ml of 1M Magnesium sulphate MgSO4 (6.02 g in 50 ml H2O) A mistake was done yesterday but this time an other did too: The last ingredients were added but they were not sterile so the medium failed again. 06-08-2015 – Experiment: Minimal and M9 medium for TPA Toxicity and BAP – VB, PV Aim: Have prepared M9 Medium and Minimal Medium for Petri dishes We restarted the last two protocols but this time we included the agar for the future Petri dishes. Solution 1: (salts) Solution 2: (agar) Solution 3: We underestimated the time for agar to solidify, so we had solid agar in an test-tube. Moreover one of the flask was not sterile so we contaminated our medium. We decided to return at the first protocol (for 1L). At the end of this journey the two media were ready but they did not have the last ingredients.
Enzymatic Assay of NB-EsteraseFigure 8: Gel de migration pour verification de la ligation. There is no band for the ligation sample. Gibson Assembly
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