Difference between revisions of "Team:Amsterdam/Project/Phy param/Synechocysytis"
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Revision as of 21:50, 17 September 2015
Synechocystis physiological parameters
Counting cells, counting molecules
Overview
Background
Estimating physiological parameters is necessary for building meaningful models that can help us to rationally design our consortium. In addition production stability can be assessed by estimating this parameters over a long period of time give.
Aim
We aim to use different cultivation strategies to Characterize growth and production rates of carbon producing Synechocystis strains their stability.
Approach
We have used turbidostat cultivation system to determine the stability of the physiological parameters
Results
- We have demonstrated the instability of classical engineering strategies. As shown in figure 1 the strain SAA023 lose the ability to produce lactate after 300 hours of continuous culture.
- We show that the Qp in the growth coupled producer Δacs remains constant over the time (figure 2).
- We have estimated Qp and growth rates for six Synechocystis (Table 1).
