Difference between revisions of "Team:Pasteur Paris/Week 10"

Line 102: Line 102:
 
               <p></p><br><br><br>
 
               <p></p><br><br><br>
 
                
 
                
<h3><em>Toxicity and  solubility of TPA (Terephthalic acid)</em></h3><br>
+
<!--<h3><em>Toxicity and  solubility of TPA (Terephthalic acid)</em></h3><br>
 
<ol>
 
<ol>
 
   <li style="text-align:left">Dissolution of TPA in DMSO (Dimethyl sulfoxide). The best concentration obtained is 62 mg/l </li>
 
   <li style="text-align:left">Dissolution of TPA in DMSO (Dimethyl sulfoxide). The best concentration obtained is 62 mg/l </li>
Line 162: Line 162:
 
<p style="text-align:left">We underestimated  the time for agar to solidify, so we had solid agar in the <strong><u>test-tube</u></strong>. Moreover one of the flask was  not sterile so we contaminated our medium. We decided to return at the first  protocol (for 1l). </p>
 
<p style="text-align:left">We underestimated  the time for agar to solidify, so we had solid agar in the <strong><u>test-tube</u></strong>. Moreover one of the flask was  not sterile so we contaminated our medium. We decided to return at the first  protocol (for 1l). </p>
 
<p style="text-align:left">At the end of  this journey the two media were ready but they did not have the last  ingredients.</p>
 
<p style="text-align:left">At the end of  this journey the two media were ready but they did not have the last  ingredients.</p>
<p style="text-align:left">&nbsp;</p>
+
<p style="text-align:left">&nbsp;</p>-->
  
 
<h3><em>Enzymatic Assay  of NB-Esterase</em></h3><br>
 
<h3><em>Enzymatic Assay  of NB-Esterase</em></h3><br>

Revision as of 01:27, 18 September 2015

Week 10

08/03 - 08/07




Enzymatic Assay of NB-Esterase


  1. XbaI ans PstI enzymatic digest of the Biobrick BBa_K808030 and pDG011 plasmid.
  2. Gel purification of the digested fragments on 0.8% agarose gel.
  3. Ligation of pDG011 plasmid and BBa_K808030 using T4 DNA ligase.
  4. Gel migration on 0.8% agarose gel.
  5. k
    Figure 8: Gel for verification of ligation.
    There is no band for the ligation sample.


    Gibson Assembly


  6. Ligation of ThpA1, Transp_, 1392932_, using T4 DNA ligase.
  7. DNA concentration measurement using a spectrophotometer.

Biobrick

DNA Concentration (ng/µl)

OD (230 nm)

OD (260 nm)

OD (280 nm)

OD (260 nm) / OD (280 nm)

OD (260 nm) / OD (230 nm)

808010

30.00

9.33

0.75

0,30

1.87

0.06

808011

50.00

11.78

1.00

0.63

1.61

0.09

808012

17.50

10.93

0.33

0.18

0.84

0.03

808013

5.00

10.55

0.10

0.06

1.56

0.01

80801v

22.5

12.13

0.47

0.30

1.54

0.04

1095000

37.50

18.48

0.78

0.43

1.83

0.04