Difference between revisions of "Team:Vilnius-Lithuania/Results"
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− | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/9/93/Vilnius15_gelis.png" style="width:450px"/> |
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− | <p style="border-left: 5px solid rgb(236,151,31); padding-left: 3px; border-bottom: none"> | + | <p style="border-left: 5px solid rgb(236,151,31); padding-left: 3px; border-bottom: none">Figure 1. Cascade restriction analysis. The wild type gene was in a pCDF vector and had one EcoRI restriction site in the gene of Cascade complex. After six mutant plasmid digestion with EcoRI, no DNA linearization was present, compared to the non-mutated Cascade control (K).</p> |
<p class="text-justify">The Cas3 gene was in a pET-Duet vector and had EcoRI, XbaI and PstI restriction sites upstream the gene (in the vector multi-cloning site area) and in the gene itself. After restriction analysis of a non-mutated Cas3 gene, two bands are visible (Figure 2). After the mutagenesis 5 out of 6 mutants were non-digestable by the enzymes.</p> | <p class="text-justify">The Cas3 gene was in a pET-Duet vector and had EcoRI, XbaI and PstI restriction sites upstream the gene (in the vector multi-cloning site area) and in the gene itself. After restriction analysis of a non-mutated Cas3 gene, two bands are visible (Figure 2). After the mutagenesis 5 out of 6 mutants were non-digestable by the enzymes.</p> |
Revision as of 06:29, 18 September 2015