Difference between revisions of "Team:Pasteur Paris/Week 10"
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<li style="text-align:left">Creation of a stock solution of soluble TPA in water at 0,5mol/L</li></br> | <li style="text-align:left">Creation of a stock solution of soluble TPA in water at 0,5mol/L</li></br> | ||
<li style="text-align:left">Test of the TPA Solubilization in LB Broth</li></br> | <li style="text-align:left">Test of the TPA Solubilization in LB Broth</li></br> | ||
− | <li style="text-align:left"><u>Preparation of different media containing (TPA) terephthalic acid and (EG) ethylene glycol:</u></br>Media 1 to 10 are made in solid form: | + | <li style="text-align:left"><u>Preparation of different media containing (TPA) terephthalic acid and (EG) ethylene glycol:</u></br>Media 1 to 10 are made in solid form:</li> |
<ol> | <ol> | ||
<li style="text-align:left">M9</li> | <li style="text-align:left">M9</li> | ||
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<li style="text-align:left">M9 + sucrose + TPA (6mM)</li> | <li style="text-align:left">M9 + sucrose + TPA (6mM)</li> | ||
<li style="text-align:left">M9 + sucrose + TPA (1,2mM)</li> | <li style="text-align:left">M9 + sucrose + TPA (1,2mM)</li> | ||
− | + | <p>Media 11 to 15 are made in both solid and liquid form:</p> | |
<li style="text-align:left">M9</li> | <li style="text-align:left">M9</li> | ||
<li style="text-align:left">M9 + TPA (1.2mM)</li> | <li style="text-align:left">M9 + TPA (1.2mM)</li> |
Revision as of 12:48, 18 September 2015
Week 1Week 2Week 3Week 4Week 5Week 6Week 7Week 8Week 9Week 10Week 11Week 12Week 13Week 14Week 15 |
Week 10 Toxicity and solubility of TPA (Terephthalic acid)
Media 11 to 15 are made in both solid and liquid form: Enzymatic Assay of NB-EsteraseFigure 8: Gel for verification of ligation. There is no band for the ligation sample. Gibson Assembly
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