Difference between revisions of "Team:Pasteur Paris/Week 14"
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<p></p><br><br><br> | <p></p><br><br><br> | ||
+ | <ol> | ||
+ | <li style="text-align:left">After 72h of incubation, only contamination was observed </li> | ||
+ | <li style="text-align:left">Co-transfection in yeast of nicked pRS415 nicked and Cre with extensions the protocol given by Heloise MÜLLER </li> | ||
+ | <li style="text-align:left">After Four days of culture of the electroporated yeast BY4742 with Cre+ homologous extension and the plasmid pRS415 nicked as vector no colonies has grown on Petri dishes.</li> | ||
+ | <ol> | ||
<p style="text-align:left">1. After 72h of incubation, only contamination was observed.</p> | <p style="text-align:left">1. After 72h of incubation, only contamination was observed.</p> | ||
<p style="text-align:left"> 2. Co-transfection in yeast of nicked pRS415 nicked and Cre with extensions the protocol given by Heloise MÜLLER. </p> | <p style="text-align:left"> 2. Co-transfection in yeast of nicked pRS415 nicked and Cre with extensions the protocol given by Heloise MÜLLER. </p> |
Revision as of 17:51, 18 September 2015
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Week 14
1. After 72h of incubation, only contamination was observed. 2. Co-transfection in yeast of nicked pRS415 nicked and Cre with extensions the protocol given by Heloise MÜLLER. 3. After Four days of culture of the electroporated yeast BY4742 with Cre+ homologous extension and the plasmid pRS415 nicked as vector no colonies has grown on Petri dishes. Assembly of Cluster 1 :
Assembly of cluster 2 : ^ |