Difference between revisions of "Team:UCLA/Notebook/Interlab Study/2 July 2015"

(Created page with " * Resuspended BioBrick plamids. **Resuspended BioBricks BBa_J23101, BBa_J23106, BBa_J23117, BBa_I3504, BBa_I270260, and BBa_R0040 in 10uL of nuclease free ddH2O, according to re...")
 
 
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* Resuspended BioBrick plamids.
 
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**Resuspended BioBricks BBa_J23101, BBa_J23106, BBa_J23117, BBa_I3504, BBa_I270260, and BBa_R0040 in 10uL of nuclease free ddH2O, according to registry instructions.  Aliquots were incubated at room temp in the wells for 5 minutes to reabsorb all dried DNA, and transferred to PCR tubes, labeled, and stored at -20 degrees Celsius.
 
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* Transformed all BioBrick plasmids in 5-alpha electrocompetent E. coli.
 
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** Conducted using the protocol listed here.
 
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** With the exception of BBa_I3504, all transformed cell lines were grown on chloramphenicol supplemented plates. (504 requires ampicillin ABX).
 
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** 200uL and 50uL aliquots of each of the 1mL rescued cells were plates using sterile glass beads on each plate, respectively.
 
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** Plates were inverted, labeled, and incubated for 16 hours in 37 degrees Celsius.
 
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*** Results of the transformations are HERE.
 
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* Resuspended BioBrick plamids.
 +
**Resuspended BioBricks BBa_J23101, BBa_J23106, BBa_J23117, BBa_I3504, BBa_I270260, and BBa_R0040 in 10uL of nuclease free ddH2O, according to registry instructions.  Aliquots were incubated at room temp in the wells for 5 minutes to reabsorb all dried DNA, and transferred to PCR tubes, labeled, and stored at -20 degrees Celsius.
 +
 +
* Transformed all BioBrick plasmids in 5-alpha electrocompetent E. coli.
 +
** Conducted using the protocol listed here.
 +
** With the exception of BBa_I3504, all transformed cell lines were grown on chloramphenicol supplemented plates. (504 requires ampicillin ABX).
 +
** 200uL and 50uL aliquots of each of the 1mL rescued cells were plates using sterile glass beads on each plate, respectively.
 +
** Plates were inverted, labeled, and incubated for 16 hours in 37 degrees Celsius.
 +
*** Results of the transformations are HERE.

Latest revision as of 01:21, 6 July 2015

  • Resuspended BioBrick plamids.
    • Resuspended BioBricks BBa_J23101, BBa_J23106, BBa_J23117, BBa_I3504, BBa_I270260, and BBa_R0040 in 10uL of nuclease free ddH2O, according to registry instructions. Aliquots were incubated at room temp in the wells for 5 minutes to reabsorb all dried DNA, and transferred to PCR tubes, labeled, and stored at -20 degrees Celsius.
  • Transformed all BioBrick plasmids in 5-alpha electrocompetent E. coli.
    • Conducted using the protocol listed here.
    • With the exception of BBa_I3504, all transformed cell lines were grown on chloramphenicol supplemented plates. (504 requires ampicillin ABX).
    • 200uL and 50uL aliquots of each of the 1mL rescued cells were plates using sterile glass beads on each plate, respectively.
    • Plates were inverted, labeled, and incubated for 16 hours in 37 degrees Celsius.
      • Results of the transformations are HERE.