Golas of module 1

• Construct a set of reporter promoters for yeast cells
• Characterize reporter promoters in all mating types
• Design synthetic MATa and MATx strains
• Build a synthetic diploid strain with a functional yeast pheromone pathway
• Demonstrate the correct functionality of yeast pheromone pathway in synthetic diploids

Goals of module 2

• Construct a set of yeast plasmids with different mating pheromones and their receptors and contribute to the Registry with new BioBricks
• Verify the correct coupling of the receptors to the yeast pheromone mating pathway
• Verify the correct expression and secretion of the different pheromones
• Show the orthogonality of the used receptors and pheromones

Goals of module 3

• Demonstrate the ability of our receptors to bind chosen markers.