Difference between revisions of "Team:Pasteur Paris/Week 15"

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<center><img src="https://static.igem.org/mediawiki/2015/6/69/IGEM-Pasteur_Gel-week-15.jpg" />
 
<center><img src="https://static.igem.org/mediawiki/2015/6/69/IGEM-Pasteur_Gel-week-15.jpg" />
<p><u><b>Figure xx:</u></b> Gel migration of the PCR  products of BBa_K936011 and BBa_K936023 on 1% agarose gel. </p></center></br>
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<p><u><b>Figure 12:</u></b> Gel migration of the PCR  products of BBa_K936011 and BBa_K936023 on 1% agarose gel. </p></center></br>
  
 
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Revision as of 23:14, 18 September 2015

Week 15

09/07 - 09/11




  • Gibson Assembly of Cluster 4

  • Figure 12: Gel migration of the PCR products of BBa_K936011 and BBa_K936023 on 1% agarose gel.


    1. Gel migration of the PCR products of BBa_K936011 and BBa_K936023 on 1% agarose gel.

    2. Gibson Assembly of cluster 4: BBa_K936011, BBa_K936023 and pSB1C3.

    3. Transformation in chemically competent E. coli DH5-α cells

    4. MiniPrep

    5. Preparation for sequencing.

  • Stock of the plasmids containing BBa_K936011, BBa_K936023, BBa_K1392932, BBa_K1392931:

    1. Transformation in chemically competent E. coli DH5-α cells.


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