Difference between revisions of "Team:Czech Republic/Parts"
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** GFP under the control of constitutive pADH1 promoter | ** GFP under the control of constitutive pADH1 promoter | ||
* pasCYC1v3_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690007 BBa_K1690007] | * pasCYC1v3_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690007 BBa_K1690007] | ||
| − | ** GFP under the control of constitutive pasCYC1v3 promoter | + | ** GFP under the control of a MATa specific constitutive pasCYC1v3 promoter |
* pTv3_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690008 BBa_K1690008] | * pTv3_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690008 BBa_K1690008] | ||
** GFP under the control of repressible pTet promoter | ** GFP under the control of repressible pTet promoter | ||
{{:Team:Czech_Republic/Template:Bottom}} | {{:Team:Czech_Republic/Template:Bottom}} | ||
Latest revision as of 01:25, 19 September 2015
Parts
Description
We relied heavily on two promoters from the Registry (pGAL1 and pCUP1), and so we extensively characterized them both and submitted the results to the Registry as a part of the Gold medal requirements.
Basic Parts
Several Basic parts were constructed during the summer. We contributed to the Registry with 8 different basic parts, ready to use in Saccharomyces cerevisiae. Function of all these parts was experimentally validated.
We sent 4 different mating pheromones:
- Mating pheromone (S. cerevisiae) - [http://parts.igem.org/Part:BBa_K1690000 BBa_K1690000]
- Mating pheromone (C. albicans) - [http://parts.igem.org/Part:BBa_K1690001 BBa_K1690001]
- Mating pheromone (C. tropicalis) - [http://parts.igem.org/Part:BBa_K1690002 BBa_K1690002]
- Mating pheromone (L. elongisporus) - [http://parts.igem.org/Part:BBa_K1690003 BBa_K1690003]
And with the sequences of 2 different pheromone receptors:
- Pheromone receptor (C. albicans) - [http://parts.igem.org/Part:BBa_K1690004 BBa_K1690004]
- Pheromone receptor (C. parapsilosis) - [http://parts.igem.org/Part:BBa_K1690005 BBa_K1690005]
The BioBricks above were constructed by Module 2, where you can find details of their construction and function.
Also, 3 different reporters ready to use in Saccharomyces were prepared and sent:
- pADH1_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690006 BBa_K1690006]
- pasCYC1v3_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690007 BBa_K1690007]
- pTv3_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690008 BBa_K1690008]
These BioBricks were constructed by Module 1, where you can find details of their construction and function.
Part Collection
Our submitted parts can be divided into two separate collections:
Signals & Receptors
- Mating pheromone (S. cerevisiae) - [http://parts.igem.org/Part:BBa_K1690000 BBa_K1690000]
- Mating pheromone (C. albicans) - [http://parts.igem.org/Part:BBa_K1690001 BBa_K1690001]
- Mating pheromone (C. tropicalis) - [http://parts.igem.org/Part:BBa_K1690002 BBa_K1690002]
- Mating pheromone (L. elongisporus) - [http://parts.igem.org/Part:BBa_K1690003 BBa_K1690003]
- Pheromone receptor (C. albicans) - [http://parts.igem.org/Part:BBa_K1690004 BBa_K1690004]
- Pheromone receptor (C. parapsilosis) - [http://parts.igem.org/Part:BBa_K1690005 BBa_K1690005]
Yeast reporters
- pADH1_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690006 BBa_K1690006]
- GFP under the control of constitutive pADH1 promoter
- pasCYC1v3_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690007 BBa_K1690007]
- GFP under the control of a MATa specific constitutive pasCYC1v3 promoter
- pTv3_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690008 BBa_K1690008]
- GFP under the control of repressible pTet promoter