Difference between revisions of "Team:Vilnius-Lithuania/Results"
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<p class="text-justify">For the fluorescence measurement experiments we took advantage of protocol that was provided by the iGEM headquarters for the InterLab Measurement experiment. We transformed JM109 bacteria with our new constructs that were cloned into two separate compatible pasmid vectors. In theory, our regulatory unit upon induction with IPTG should produce cI repressor, which, in turn, should downregulate the expression of the GFP gene (under the control of cI regulated promoter). We seeked to quantify this by growing cell cultures overnight with different IPTG concentrations. We also used a negative and a positive control. </p> | <p class="text-justify">For the fluorescence measurement experiments we took advantage of protocol that was provided by the iGEM headquarters for the InterLab Measurement experiment. We transformed JM109 bacteria with our new constructs that were cloned into two separate compatible pasmid vectors. In theory, our regulatory unit upon induction with IPTG should produce cI repressor, which, in turn, should downregulate the expression of the GFP gene (under the control of cI regulated promoter). We seeked to quantify this by growing cell cultures overnight with different IPTG concentrations. We also used a negative and a positive control. </p> | ||
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<p class="text-justify">Here are the results:</p> | <p class="text-justify">Here are the results:</p> |
Revision as of 19:00, 1 October 2015