Difference between revisions of "Team:UCLA/Notebook/Recombinant Expression/6 July 2015"

Line 290: Line 290:
 
Calculations of Reagent Quantities Needed  
 
Calculations of Reagent Quantities Needed  
 
We account for inflation of cell pellet weight due to inclusion of Falcon tube mass, and the reagents are added in the following ratios:
 
We account for inflation of cell pellet weight due to inclusion of Falcon tube mass, and the reagents are added in the following ratios:
 +
 
Lysozyme: 0.2 mg/mL of solution
 
Lysozyme: 0.2 mg/mL of solution
 +
 
PMSF Solution: added to final concentration of 1mM
 
PMSF Solution: added to final concentration of 1mM
 +
 
Deoxycholic Acid: 1.5 g/g of cell pellet
 
Deoxycholic Acid: 1.5 g/g of cell pellet
  

Revision as of 00:21, 10 July 2015

Cell Lysis, continued

Calculations of Reagent Quantities Needed We account for inflation of cell pellet weight due to inclusion of Falcon tube mass, and the reagents are added in the following ratios:

Lysozyme: 0.2 mg/mL of solution

PMSF Solution: added to final concentration of 1mM

Deoxycholic Acid: 1.5 g/g of cell pellet

Mass of Cell Pellet Lysozyme PMSF Solution Deoxycholic Acid
0.52g 0.0082g 0.41mL 0.78g
0.32g 0.0082g 0.41mL 0.48g
0.42g 0.0084g 0.42mL 0.63g
0.52g 0.0084g 0.42mL 0.78g
0.42g 0.0084g 0.42mL 0.63g
0.52g 0.0084g 0.42mL 0.78g
0.02g 0.0084g 0.42mL 0.03g
0.52g 0.0084g 0.42mL 0.78g
0.82g 0.0084g 0.42mL 1.23g
0.32g 0.0084g 0.42mL 0.48g
0.32g 0.0084g 0.42mL 0.48g
0.42g 0.0084g 0.42mL 0.63g
0.22g 0.0084g 0.42mL 0.33g
0.52g 0.0084g 0.42mL 0.78g
0.62g 0.0084g 0.42mL 0.93g
0.42g 0.0084g 0.42mL 0.63g
0.32g 0.0084g 0.42mL 0.48g
0.12g 0.0084g 0.42mL 0.18g
0.62g 0.0084g 0.42mL 0.93g
0.52g 0.0084g 0.42mL 0.78g