Difference between revisions of "Team:UCLA/Notebook/Recombinant Expression/6 July 2015"

Revision as of 00:23, 10 July 2015

Cell Lysis, continued

Calculations of Reagent Quantities Needed for All 20 Samples

We account for inflation of cell pellet weight due to inclusion of Falcon tube mass, and the reagents are added in the following ratios:

Lysozyme: 0.2 mg/mL of solution

PMSF Solution: added to final concentration of 1mM

Deoxycholic Acid: 1.5 g/g of cell pellet

DNAse: 3 ul added to each sample

Mass of Cell Pellet	Lysozyme	PMSF Solution	Deoxycholic Acid
0.52g	0.0082g	0.41mL	0.78g
0.32g	0.0082g	0.41mL	0.48g
0.42g	0.0084g	0.42mL	0.63g
0.52g	0.0084g	0.42mL	0.78g
0.42g	0.0084g	0.42mL	0.63g
0.52g	0.0084g	0.42mL	0.78g
0.02g	0.0084g	0.42mL	0.03g
0.52g	0.0084g	0.42mL	0.78g
0.82g	0.0084g	0.42mL	1.23g
0.32g	0.0084g	0.42mL	0.48g
0.32g	0.0084g	0.42mL	0.48g
0.42g	0.0084g	0.42mL	0.63g
0.22g	0.0084g	0.42mL	0.33g
0.52g	0.0084g	0.42mL	0.78g
0.62g	0.0084g	0.42mL	0.93g
0.42g	0.0084g	0.42mL	0.63g
0.32g	0.0084g	0.42mL	0.48g
0.12g	0.0084g	0.42mL	0.18g
0.62g	0.0084g	0.42mL	0.93g
0.52g	0.0084g	0.42mL	0.78g

@@ Line 288: / Line 288: @@
 <u>Cell Lysis, continued</u>
-Calculations of Reagent Quantities Needed
+Calculations of Reagent Quantities Needed for All 20 Samples
 We account for inflation of cell pellet weight due to inclusion of Falcon tube mass, and the reagents are added in the following ratios:
-Lysozyme: 0.2 mg/mL of solution
+'''Lysozyme:''' 0.2 mg/mL of solution
 PMSF Solution: added to final concentration of 1mM
 Deoxycholic Acid: 1.5 g/g of cell pellet
+DNAse: 3 ul added to each sample
 {| class="wikitable"