Difference between revisions of "Team:UCLA/Notebook/Recombinant Expression/10 July 2015"
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| + | We continue troubleshooting our batch purification protocol, this time trying it out on another sample of our lysate. We did notice that yesterday, when we first diluted our Lysis Buffer in 1X Binding buffer in a 1:1 volume ratio, coagulation and precipitation appeared. As the contents of the tube came closer to room temperature, coagulation and precipitation lessened, but still present. We suspect that the lysate sample used yesterday had excess contaminants that led to this unexpected precipitation. Today, when diluting our lysate sample in 1X Binding buffer, again in a 1:1 volume ratio, the | ||
Revision as of 22:16, 10 July 2015
Batch Purification, continued
We continue troubleshooting our batch purification protocol, this time trying it out on another sample of our lysate. We did notice that yesterday, when we first diluted our Lysis Buffer in 1X Binding buffer in a 1:1 volume ratio, coagulation and precipitation appeared. As the contents of the tube came closer to room temperature, coagulation and precipitation lessened, but still present. We suspect that the lysate sample used yesterday had excess contaminants that led to this unexpected precipitation. Today, when diluting our lysate sample in 1X Binding buffer, again in a 1:1 volume ratio, the