Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/13 July 2015"
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Revision as of 21:42, 13 July 2015
Colony PCR of 7/12 Transformation
- The transformation was successful for the 3:1 ligated products plates. I picked 3 colonies off the plate and suspended each in 100uL of ddH2O.
- Using APEX Taq Red Mastermix
| Component | Volume (out of 25L) |
|---|---|
| APEX Taq Red Mastermix | 12.5uL |
| 10mM primer 10 | 1.25uL |
| 10mM primer 11 | 1.25uL |
| Transformed Cells | 1uL |
| Nuclease Free Water | 9uL |