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| − | Intro: Miniprep Yeates' wt cage cells. Assisted Phil in making reagents. Lab meeting. Prepared protocols for transformation.
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| − | Miniprep Used 2mL of 10mL overnight starter culture. Used highest centrifuge setting for all centrifugations. Yield: 108.01 ng/uL 1.98 260/280. stored in -20C boelter protein cage box.
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| − | Notes: Remember to put cap on centrifuge. Rcf dorresponds to g's. Switch between using rpm/rcf button. Balance all tubes with 1.5mL tube and water. Not super precise but its ok. The P3 buffer is in the fridge with the glass door.
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| − | Solution making technique: Rotate solid reagent bottle to pout out precise amount. Add acid/base to water. If no flat surface, hold bottle with two fingers to level it.
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| − | Lab meeting notes: Think of types of people to contact for human practices. Wearable biomaterials. iGEM journal club next Wednesday.
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| − | Michael Gel making: Mix agar and TAE buffer. Microwave for a couple minutes. Let it boil for about 10 seconds before stopping microwave. Add cybrsafe. All casting trays/electrophoresis machines in 7731.
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| − | Tyler Lee --[[User:Wtleeiv|Wtleeiv]] 22:46, 14 July 2015 (CDT)
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