Difference between revisions of "Team:UCLA/Notebook/Protein Cages/13 July 2015"

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Intro: Miniprep Yeates' wt cage cells. Assisted Phil in making reagents. Lab meeting. Prepared protocols for transformation.  
 
Intro: Miniprep Yeates' wt cage cells. Assisted Phil in making reagents. Lab meeting. Prepared protocols for transformation.  
  

Revision as of 03:51, 15 July 2015

iGEM UCLA




Intro: Miniprep Yeates' wt cage cells. Assisted Phil in making reagents. Lab meeting. Prepared protocols for transformation.

Miniprep Used 2mL of 10mL overnight starter culture. Used highest centrifuge setting for all centrifugations. Yield: 108.01 ng/uL 1.98 260/280. stored in -20C boelter protein cage box.

Notes: Remember to put cap on centrifuge. Rcf dorresponds to g's. Switch between using rpm/rcf button. Balance all tubes with 1.5mL tube and water. Not super precise but its ok. The P3 buffer is in the fridge with the glass door.

Solution making technique: Rotate solid reagent bottle to pout out precise amount. Add acid/base to water. If no flat surface, hold bottle with two fingers to level it.

Lab meeting notes: Think of types of people to contact for human practices. Wearable biomaterials. iGEM journal club next Wednesday.

Michael Gel making: Mix agar and TAE buffer. Microwave for a couple minutes. Let it boil for about 10 seconds before stopping microwave. Add cybrsafe. All casting trays/electrophoresis machines in 7731.

Tyler Lee --Wtleeiv 22:46, 14 July 2015 (CDT)