Difference between revisions of "Team:UCLA/Notebook/Recombinant Expression/23 July 2015"
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'''<u>IMAC Purification, Take Two</u>''' | '''<u>IMAC Purification, Take Two</u>''' | ||
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| + | 2mL of fresh resin was added to the a pre-chilled 50mL Falcon tube. The contents were centrifuged to discard the ethanol supernatant. The standard IMAC Batch Purification protocol was followed, as in previous purification trials. | ||
Revision as of 20:23, 24 July 2015
IMAC Purification, Take Two
2mL of fresh resin was added to the a pre-chilled 50mL Falcon tube. The contents were centrifuged to discard the ethanol supernatant. The standard IMAC Batch Purification protocol was followed, as in previous purification trials.