Difference between revisions of "Team:UCLA/Notebook/Protein Cages/24 July 2015"
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| − | [[Media:IMG_2602.JPG]] | + | Intro: Second round of PCR to add on iGEM suffix. Ran on agarose gel to confirm. Didn't work (300 bp bands). Sad day :( |
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| + | PCR: Gradient PCR at temps of 58, 62, 66, and 70 C using Q5 polymerase. Followed NEB Q5 protocol. 2.5 uL of 2 mM dNTP's. 0.25 uL of 1 ng/uL cage template. 14.5 uL water per reaction. | ||
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| + | Agarose Gel: Used gel Phil made that was stored in 7731 fridge. Leftmost lane (#1) generuler 1 kb ladder. Lane 2 empty. Lanes 3-6 58, 62, 66, 70 C from left to right. [[Media:IMG_2602.JPG]] | ||
Revision as of 23:19, 24 July 2015
Intro: Second round of PCR to add on iGEM suffix. Ran on agarose gel to confirm. Didn't work (300 bp bands). Sad day :(
PCR: Gradient PCR at temps of 58, 62, 66, and 70 C using Q5 polymerase. Followed NEB Q5 protocol. 2.5 uL of 2 mM dNTP's. 0.25 uL of 1 ng/uL cage template. 14.5 uL water per reaction.
Agarose Gel: Used gel Phil made that was stored in 7731 fridge. Leftmost lane (#1) generuler 1 kb ladder. Lane 2 empty. Lanes 3-6 58, 62, 66, 70 C from left to right. Media:IMG_2602.JPG
