Difference between revisions of "Team:UCLA/Notebook/Protein Cages/31 July 2015"
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| − | {{ | + | {{Template:UCLA}} |
Nithin's Notes | Nithin's Notes | ||
| − | I ran a touchdown pcr today. | + | I ran a touchdown pcr today. There is a strong band where we want it and a weak nonspecific band. Will upload pic tmro. |
| + | |||
| + | Prefix 1 and Suffix 1 with PCquad. | ||
Conditions: | Conditions: | ||
Latest revision as of 01:39, 1 August 2015
Nithin's Notes
I ran a touchdown pcr today. There is a strong band where we want it and a weak nonspecific band. Will upload pic tmro.
Prefix 1 and Suffix 1 with PCquad.
Conditions:
- 1-cycle-
- 98 degrees- 30s
- 10-cycles
- - 98 degrees- 10s
- - 76-66 degrees (decrease by 1 degree each cycle)-30 seconds
- - 72 degrees- 15s
- 15-cycles
- - 98 degrees- 10s
- - 66 degrees- 30s
- - 72 degrees- 15s
- 1-cycle
- - 72 degrees- 5 minute
- - 4 degree hold.