Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics/12 August 2015"
Oliviacheng (Talk | contribs) (→Colony PCR for M1 SwqAB 1 & 2 and M1-9(T7)) |
Oliviacheng (Talk | contribs) (→Colony PCR for M1 SwqAB 1 & 2 and M1-9(T7)) |
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[[File:ColonyPCR0812.jpg |800px|thumb|left|Lane 3-6: M1-9(T7) Colony 1-4. Lane 7-10: M1SeqAB1 Colony 1-4. Lane 11-14: M1SeqAB2 Colony 1-4]] | [[File:ColonyPCR0812.jpg |800px|thumb|left|Lane 3-6: M1-9(T7) Colony 1-4. Lane 7-10: M1SeqAB1 Colony 1-4. Lane 11-14: M1SeqAB2 Colony 1-4]] | ||
| + | |||
| + | Grew liquid culture for | ||
| + | * all 4 colonies of M1-9 (T7); they all showed band close to the expected size ~1200-1300bp but they are slightly different so decided to sequence all of them. | ||
| + | * colonies 1-3 for M1SeqAB1 | ||
| + | * none for M1SeqAB2 since we probably do not need this seq construct | ||
Revision as of 06:37, 13 August 2015
08/12/2015
Colony PCR for M1 SwqAB 1 & 2 and M1-9(T7)
| 2X Taq MM | 6.25 uL |
| VF Primer | 0.625 uL |
| VR Primer | 0.625 uL |
| Template | 1 uL |
| ddH2O | 4 uL |
| 95C | 3 min |
| 95C | 30 sec |
| 72C | 25 sec (M1SeqAB)/ 1min 40 sec (M1-9) |
| x30 | |
| 72C | 5 min |
| 12C | Hold |
Grew liquid culture for
- all 4 colonies of M1-9 (T7); they all showed band close to the expected size ~1200-1300bp but they are slightly different so decided to sequence all of them.
- colonies 1-3 for M1SeqAB1
- none for M1SeqAB2 since we probably do not need this seq construct