Team:UCLA/Notebook/Honeybee Silk/18 August 2015

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Transformation with the Silk+GFP Construct

• Using the new BL21 cells that I made last week

1. Thaw cells (50uL) on ice for 10 minutes.
2. Add 1uL of ligated product.
3. Place on ice for 5 minutes.
4. Rescue in 200uL of SOC, incubate and shake for 1 hour.
5. Warm chloramphenicol plates at 37C.
6. Spin down bacteria to pellet, discard supernatant.
7. Make 2 serial dilutions (1:10 and 1:100)
8. Plate 100uL of bacteria (1:1, 1:10, 1:100) on each plate.
9. Spread with beads, incubate at 37C.