Team:UCLA/Notebook/Spider Silk Genetics/Protocols/Iterative Capped Assembly

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This document details steps to perform ICA for Spider Silk Gene Assembly.

ICA Preparation

• Required Solutions:
  • 2x BW Buffer
  • 0.5x BW Buffer
  • Elution Buffer
• Prepared, working solutions of the Initator, Terminator and Capping Oligos
• Purified, BsaI Digested MaSp Monomers
  • Sufficient amount to complete ICA for the desired chain length.
  • 50 ng monomer is used in each extension reaction.