Team:UCLA/Notebook

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Notebook Template

mm/dd - mm/dd (most recent weeks on top)

mm/dd: Entry title/short description (most recent days on top)

Plain text for descriptions, etc.

Unordered lists for bullet points

• Bulletpoint 1
• Bulletpoint 2
• Bulletpoint 3

For images, upload image onto wiki server. Then replace placeholder image url with your image's url.

Ordered lists for numbers

1. Number 1
2. Number 2
3. Number 3

For tables, "tr" stands for "table row", and "td" means "table column."

Row 1, Column 1	Row 1, Column 2	Row 1, Column 3	Row 1, Column 4
Row 2, Column 1	Row 2, Column 2	Row 2, Column 3	Row 2, Column 4
Row 1, Column 1	Row 2, Column 2	Row 3, Column 3	Row 4, Column 4

For different types of text styling... bold, italics, underlined. For headers...

Headers

Smaller Header

You get the point

Feel free to google any other styling.

Keep all your html within the detail tags for the day/week you're putting your entry for.

To properly indent your html code, use: http://www.freeformatter.com/html-formatter.html

mm/dd: Sample Entry

Today we began cloning our GFP.

• PCR'd off template
• Ran gel
• Restriction digest
• Ligated into backbone

For our ligation, we made the following modifications:

1. Tried it with newly bought ligase
2. Left reaction overnight instead of 2 hrs
3. Vector to insert ratio was 1:5 instead of 1:3

PCR Reaction:

Component	Volume
5X Q5 Reaction Buffer	5
10 mM dNTPs	0.5
10 uM Forward (primer 3/7)	1.25
10 uM Reverse (primer 8)	1.25
Template (diluted to 1ng/uL)	0.5
Q5 High Fidelity DNA Polymerase	0.25
Nuclease Free Water	16.25

Gel: Lot of bands, all at correct sizes

mm/dd - mm/dd (most recent weeks on top)

mm/dd: Entry title/short description (most recent days on top)

Plain text for descriptions, etc.

Unordered lists for bullet points

• Bulletpoint 1
• Bulletpoint 2
• Bulletpoint 3

For images, upload image onto wiki server. Then replace placeholder image url with your image's url.

Ordered lists for numbers

1. Number 1
2. Number 2
3. Number 3

For tables, "tr" stands for "table row", and "td" means "table column."

Row 1, Column 1	Row 1, Column 2	Row 1, Column 3	Row 1, Column 4
Row 2, Column 1	Row 2, Column 2	Row 2, Column 3	Row 2, Column 4
Row 1, Column 1	Row 2, Column 2	Row 3, Column 3	Row 4, Column 4

For different types of text styling... bold, italics, underlined. For headers...

Headers

Smaller Header

You get the point

Feel free to google any other styling.

Keep all your html within the detail tags for the day/week you're putting your entry for.

To properly indent your html code, use: http://www.freeformatter.com/html-formatter.html

mm/dd: Sample Entry

Today we began cloning our GFP.

• PCR'd off template
• Ran gel
• Restriction digest
• Ligated into backbone

For our ligation, we made the following modifications:

1. Tried it with newly bought ligase
2. Left reaction overnight instead of 2 hrs
3. Vector to insert ratio was 1:5 instead of 1:3

PCR Reaction:

Component	Volume
5X Q5 Reaction Buffer	5
10 mM dNTPs	0.5
10 uM Forward (primer 3/7)	1.25
10 uM Reverse (primer 8)	1.25
Template (diluted to 1ng/uL)	0.5
Q5 High Fidelity DNA Polymerase	0.25
Nuclease Free Water	16.25

Gel: Lot of bands, all at correct sizes

mm/dd - mm/dd (most recent weeks on top)

mm/dd: Entry title/short description (most recent days on top)

Plain text for descriptions, etc.

Unordered lists for bullet points

• Bulletpoint 1
• Bulletpoint 2
• Bulletpoint 3

For images, upload image onto wiki server. Then replace placeholder image url with your image's url.

Ordered lists for numbers

1. Number 1
2. Number 2
3. Number 3

For tables, "tr" stands for "table row", and "td" means "table column."

Row 1, Column 1	Row 1, Column 2	Row 1, Column 3	Row 1, Column 4
Row 2, Column 1	Row 2, Column 2	Row 2, Column 3	Row 2, Column 4
Row 1, Column 1	Row 2, Column 2	Row 3, Column 3	Row 4, Column 4

For different types of text styling... bold, italics, underlined. For headers...

Headers

Smaller Header

You get the point

Feel free to google any other styling.

Keep all your html within the detail tags for the day/week you're putting your entry for.

To properly indent your html code, use: http://www.freeformatter.com/html-formatter.html

mm/dd: Sample Entry

Today we began cloning our GFP.

• PCR'd off template
• Ran gel
• Restriction digest
• Ligated into backbone

For our ligation, we made the following modifications:

1. Tried it with newly bought ligase
2. Left reaction overnight instead of 2 hrs
3. Vector to insert ratio was 1:5 instead of 1:3

PCR Reaction:

Component	Volume
5X Q5 Reaction Buffer	5
10 mM dNTPs	0.5
10 uM Forward (primer 3/7)	1.25
10 uM Reverse (primer 8)	1.25
Template (diluted to 1ng/uL)	0.5
Q5 High Fidelity DNA Polymerase	0.25
Nuclease Free Water	16.25

Gel: Lot of bands, all at correct sizes