Team:UCLA/Notebook/Spider Silk Genetics/27 April 2015

iGEM UCLA

{:Team:UCLA/CSS}}

4/27/2015

1 2AB, 2BC, 2CA Sticky End Ligation Test

	AB+BC	BC+CA	CA+AB	AB+BC+CA
2x T7 Ligase Buffer	5 uL	5 uL	5 uL	5 uL
ddH2O	1.5 uL	1.5 uL	0.5 uL	0 uL
DNA 1	2 uL	1 uL	2 uL	2 uL
DNA 2	1 uL	2 uL	2 uL	1 uL
DNA 3	n/a	n/a	n/a	2 uL
T7 Ligase	0.5 uL	0.5 uL	0.5 ul	0.5 uL
Total	10 uL	10 uL	10 uL	10 uL

25 C	5 min
65 C

∙ The ligation test was run on 2% gel with 2 uL of 50 bp ladder. Results shown below.

Figure 1: T7 Ligation Test with MaSp2 fragments. BC was used as a negative ligation control. The presence of ladders may indicate non-specific binding.