iGEM UCLA

ICA Primers

	Sequence	Length (bp)
Post-Elution Primer (Forward)	5'-GAA TTC GCG GCC GCT TC-3'	17
Post-Elution Primer (Reverse)	5'-CAG TCT GCA GCG GCC G-3'	16

ICA Oligos Sequences (Val sticky ends)

	Sequence	Length
Initiator-F	/52-Bio/actggaattcgcggccgcttctagATGcatcatcaccatcaccaccatcataagcttgc	59
Initiator-A-R	/5Phos/AACTgcaagcttatgatggtggtgatggtgatgatgCATctagaagcggccgcgaattc	59
Terminator-A-F	/5Phos/AGTTggatcctaataaTACTAGTAGCGGCCGCTGCAGACTG	41
Terminator-R	CAGTCTGCAGCGGCCGCTACTAGTAttattaggatcc	37
A-cap	/5Phos/agttGACCCGTCACGCTCGAGTATCGTAACTCGAGCGTGACGGGTC	46
B-cap	/5Phos/tgtcGACCCGTCACGCTCGAGTATCGTAACTCGAGCGTGACGGGTC	46
C-cap	/5Phos/cgtgGACCCGTCACGCTCGAGTATCGTAACTCGAGCGTGACGGGTC	46