Team:UCLA/Notebook/Recombinant Expression/29 May 2015

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• Starter Culture Failure #3 : Back in Black
  • Starter cultures grown on Wednesday were taken from the incubator at 1300 hours (~48 hours) and OD600 was taken on the samples.
    • Tamura Plate 3 -- OD600: 1.273, Tamura Plate 4-- OD600: 1.246, Tamura Plate 5: OD600: 1.279.

• Plasmid was mini prepped using the Promega PureYield Plasmid Miniprep System

• Plasmid ran on gel to verify size of plasmid, sequenced to verify proper insert, the will be used to transform into BL21(DE3) cells.

Julian's Work

• I inoculated a 500mL culture in LB with Chlor at a 1000X dilution. I used a 5mL starter culture grown overnight also in LB. I am measuring the OD every hour or so. After the first hour it was onlt 0.02 so I will let it grow for longer before inoculating.
  • This culture will be used to make the hydrogel assays.