Team:UCLA/Notebook/Honeybee Silk/4 September 2015

• Home
• Team
  • Meet the Team
  • Attributions
  • Collaborations
• Projects
  • Programming Spider Silk
  • Functionalizing Silk
  • Honeybee Silk
  • Protein Expression and Processing
  • Protein Cages
  • Human Practices
  • Interlab Study
• Parts
  • Team Parts
  • Basic Parts
  • Composite Parts
  • Part Collection
• Notebook
  • Programming Spider Silk
  • Functionalizing Silk
  • Honeybee Silk
  • Materials Processing
  • Protein Cages
  • Interlab Study
• Safety
• Judging

SDS PAGE

• Running SDS PAGE in order to assess different lysis protocols
• The gel is running weird. The reason may be that the new gels we received from MArk Arbing require a special running buffer, and I used the Glycine running buffer that we have.
• Lanes
  • {| class="wikitable"

! Lane ! Sample |- | 1 | BSA positive control |- | 2 | Negative control (water) |- | 3 | Philip's Lysis Protocol |- | 4 | Philip Lysis Full Cell Lysate (FCL) |- | 5 | Fasih's Lysis |- | 6 | Fasih' FCL |- | 7 | Bio rad dual color ladder |- | 8 | Fasigh's lysis (no iptg) |- | 9 | 1X concentrated silk |- | 10 | 3X Concentrated silk |- | 11 | Silk concentration flow through |- | 14 | Bug buster lysis |}