iGEM UCLA

8/27/2015

Sequencing Results

M1-12(1C3)
- 1: wrong sequence entirely
- 2: wrong sequence entirely
- 3: wrong sequence entirely
- Interestingly, all three sequences align to each other.
M1/2[1:1]-12(1C3)
- 1: Bad, single base pair mutation at ~550
- 2: GOOD
- 3: GOOD
M1/2[1:1]-12(T7)
- 1: Bad, deletion at ~80
- 2: GOOD
- 3: possibly correct, but not trustworthy due to short read.
M1/2[2:1]-12(1C3)
- 1: Bad, single bp mutation near ~660
- 2: Bad, single bp mutation at 962
- 3: GOOD
M1/2[2:1]-12(T7)
- 1: bad, non-silent single bp mutation at 1004
- 2: GOOD? silent single bp mutation at 702
- 3: GOOD? silent single bp mutation at 702