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Week 2
06/08 - 06/12
- Not I enzymatic digest of BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003.
- Gel migration of the Miniprep (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003)
Figure 1: Verification of the plasmid purification.
|
316003 |
808007 |
808010 |
808011 |
808012 |
808013 |
808014 |
936000 |
Theoretical size of insert(pb) |
1061 |
4368 |
948 |
1011 |
1242 |
465 |
1506 |
885 |
Present inserts match to theoretical values |
no |
yes |
C1/C2 yes
C3 no |
yes |
no |
no |
yes |
no |
Figure 2: Table of the migration gel done previously (cf. figure 2).
- Storage at -20°C of BBa_808007, BBa_808014, BBa_808011 (only for C2/C3)
- Plasmid purification of BBa_316003, BBa_808010, BBa_808012, BBa_808013, BBa_936000.
BB |
clone 1 |
clone 2 |
clone 3 |
C (ng/µL) |
260/280 |
260/230 |
C (ng/µL) |
260/280 |
260/230 |
C (ng/µL) |
260/280 |
260/230 |
808007 |
22.6 |
1.99 |
2.04 |
46.9 |
1.92 |
2.09 |
32.3 |
1.9 |
1.79 |
808010 |
13.5 |
1.85 |
1.39 |
57.1 |
1.95 |
2.17 |
31.4 |
1.86 |
1.84 |
808011 |
37.4 |
1.97 |
2 |
61.2 |
1.62 |
1.16 |
21 |
1.93 |
1.72 |
808012 |
30.2 |
1.93 |
1.94 |
54.9 |
1.91 |
2.12 |
21.1 |
1.94 |
1.81 |
808013 |
11.5 |
1.91 |
1.11 |
9.6 |
1.78 |
1.42 |
13.9 |
1.74 |
1.21 |
808014 |
33.9 |
1.89 |
1.62 |
24.3 |
1.96 |
2.14 |
14.4 |
1.81 |
1.38 |
316003 |
13.6 |
2.04 |
2.38 |
16.5 |
2.1 |
2.41 |
18.8 |
1.94 |
0.74 |
936000 |
18.2 |
1.98 |
1.19 |
13.1 |
1.97 |
0.93 |
11.9 |
2.16 |
1.18 |
Figure 4: Plasmid purification for BBa_808007, BBa_808010, BBa_808011, BBa_808012, BBa_808013, BBa_808014, BBa_316003, BBa_936000.
- Plasmid purification of BBa_316003, BBa_808010, BBa_808012, BBa_808013, BBa_936000.
Figure 5: Migration gel of digestion products.
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