Team:Pasteur Paris/Week 1

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Week 1

06/01 - 06/05




  1. Resuspension of DNA in 15µL of DNAse, RNAse free water: BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003.

  2. Storage at -20°C.

  3. Transformation in DH5α Competent cells purchased from Invitrogen (# 18265-017) was done following the transformation protocol. (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003).

  4. Cells were plated on LB + chloramphenicol 34µg/mL (Cm34).

  5. Incubation overnight.

  6. Storage at 4°C.

  7. Plasmid purification following the MiniPrep protocol.

BBa

Concentration (ng/µL)

Clone 1

Clone 2

Clone 3

808007

73.4

44.3

56.3

808010

40.8

27.5

29.5

808011

28.8

30.4

18.5

808012

49.5

#VALEUR!

56.5

808013

18.8

26

15.7

808014

30

41.4

37.4

316003

51.5

23.5

36.1

936000

15.2

17.7

13.8

 



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