Team:Vilnius-Lithuania/Project
Description
We created Coliclock - a bacterial system, which helps to limit the spreading of GMOs in nature. To construct our system we used I-F type CRISPR-Cas mechanism, which contains Cascade complex, cas3 protein and crRNA genes. Click below to find out more.
Design
Coliclock was designed by creating regulatory and functional units. Regulatory unit consists of pLux/cI promoter, LuxI, LuxR, pLac operon and cI repressor. Functional unit consists of Cascade complex, Cas3 protein and crRNR genes. Click below to find out more.
Results
Coliclock is a project that is based on CRISPR-Cas system. Our goal was to use Coliclock to prevent bacteria spreading. Coliclock works without extracellular signals. Click below to find out more.