Team:KAIT Japan/Safety

Safety

  • Safe Project Design


  • Safe Lab Work


  • Safe Shipment

    • Q1.Your New Parts
    Complete the spreadsheet.Include all whole organisms that you will handle in the lab,Whether you are using them as a chasasis or for some other reason.Include all new highly modified protein coding parts that you are using.If you submitted a Check-in for an organism or part.you should still include it in this spreadsheet.
    You may amit non-protein-coding parts,and you may amit parts that were already in the Reglsry if you are using them without significant modifications.
    A:We use DH-5α E. coli by purchased from research reagent company.
    This bacteria is not harmful and classified and classified Risk group1.We use it to produce many DNA plasmid copies and protein.
    We made protein coding parts such as 145K, 145N, lac+RBS+145N,lac+RBS+MCS+145N.
    All of the parts are fluorescent proteins and those are no toxicity.

    Q2.What is your chassis organism?
    A:E.coli

    Q3:Do you plan to experiment with any other organisms,besides your chassis?
    A:None.

    Q4:How will your project work?
    A:We try to control protein activity by light using Dronpa.By using this system,we would like to control cell devision and metabolism of E.coli,detect a pollutant.
    In this project ,we try to control the Iuciferase activity by the recombinant Dronpa as a model case.Our recombinant Dronpa Change the conformation and form tetramer by 400nm light and dissociate to monomer by 500nm light.We would like to apply the Dronpa by making the fusion luciferase with the Dronpa and the activity will be controlled by light because the protein conformation will depends on the formation of Dronpa.This control system by using Dronpa will be applicable to many enzyme.

    Q5:What risks does your project pose at the laboratry stage?What actions are you taking to reduce these risks?
    A:We put on white robe, rubber gloves to preventus from getting hurt.
    We have sterilized our used materials by autoclave, ethanol.We have experimented in clean bench to prevent our organism from escaping from lab.

    Q6:How would your project be used in the real world?
    A:
    ・Our project is foundational/we do not have a specific real-world application in mind.
    (examples:library of standardzed promoters.system for communication between cells)
    ・Only in the lab.
    (examples:reporter strain for measuring the strength of promoters)
    ・In a consumer produst that ordinary peoplebuy.
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