Safety
So we stay in one piece.
Chassis Organisms
We genetically modified Escherichia coli and Lactococcus lactis in our project. The genes were constructed in E. coli and transformed into Lactococcus lactis using a shuttle vector known as pTRKH2. Both organisms are level 1 (low risk) safety level organisms.
Safety Training
Our safety training was performed by Utah State University Environmental Health and Safety Office and covered chemical hygiene principles, spill prevention, hazardous waste management, and fire safety.
Laws and Regulations that Govern Biosafety in Research Laboratories in Our Country
Information concerning relevant laws and regulations in the United States of America may be found on the U.S. Department of Health and Human Services website at http://www.phe.gov/s3/legal/Pages/laws.aspx.
Risks Posed at the Laboratory Stage and Actions Taken to Reduce Risks
How Our Project Would Be Used in the Real World
Future Risks If Our Project Was Fully Developed and Steps That Could Be Taken to Reduce Risks
There is a relatively low level of risk from the bacteria themselves, even though they would be used in food production. The primary concern that may arise is the fact that the bacteria have an antibiotic resistance (erythromycin) in the vector plasmid used to transform our construct into the bacteria. That antibiotic resistance could potentially spread to other bacteria. Since erythromycin is primarily used in treating acne, it's unlikely that the Lactococcus lactis used in cheese culture will come into contact with bacteria causing acne on human skin enough to spread the resistance. Further work that could be done to reduce risk further might be work with a cre/lox system in the plasmid to remove the antibiotic resistance altogether after the plasmid is known to be in the cells.
