Team:KAIT Japan/Description

Project


 
 In this year, we use the fluorescent protein, “Dronpa“, to make protein regulation system. Dronpa has photochromism, Dronpa able to change its state bright from dark or dark from bright. Dronpa normally absorbs at 503 nm and emits green fluorescence, strong irradiation at 488 nm can convert this protein to a nonfluorescent state that absorbs at 390 nm. The protein can then be switched back to the original emissive state with minimal irradiation at 405 nm.
 We use Dronpa145N which is Lys145Asn mutant of Dronpa. At the bright state, this protein forms tetramer. While at dark state, Dronpa145N form monomer. The ensemble absorption and mission properties of Dronpa145N are highly similar to those of Dronpa. We utilize this conformation change for Light-dependent regulation of flexibility in a protein.