Team:Pasteur Paris/Week 10

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Week 10 08/03 - 08/07 Enzymatic Assay of NB-Esterase XbaI ans PstI enzymatic digest of the Biobrick BBa_K808030 and pDG011 plasmid. Gel purification of the digested fragments on 0.8% agarose gel. Ligation of pDG011 plasmid and BBa_K808030 using T4 DNA ligase. Gel migration on 0.8% agarose gel. Figure 8: Gel for verification of ligation. There is no band for the ligation sample. Gibson Assembly Ligation of ThpA1, Transp_, 1392932_, using T4 DNA ligase. DNA concentration measurement using a spectrophotometer. Biobrick DNA Concentration (ng/µl) OD (230 nm) OD (260 nm) OD (280 nm) OD (260 nm) / OD (280 nm) OD (260 nm) / OD (230 nm) 808010 30.00 9.33 0.75 0,30 1.87 0.06 808011 50.00 11.78 1.00 0.63 1.61 0.09 808012 17.50 10.93 0.33 0.18 0.84 0.03 808013 5.00 10.55 0.10 0.06 1.56 0.01 80801v 22.5 12.13 0.47 0.30 1.54 0.04 1095000 37.50 18.48 0.78 0.43 1.83 0.04