Team:KAIT Japan/Results

Results


[Expression of Dronpa 145K and Dronpa 145N]
We were cloning Dronpa 145K and Dronpa 145N in E.coli. And we expressed these parts using pcold vector.



E.coli fluorescence when we irradiated about 400 nm.



[Purification of Dronpa 145N and 145K]
We purified Dronpa 145N and Dronpa 145K by His-tag purification.



We irradiated about 400 nm at purification of Dronpa 145N. It fluorescence green.



[SDS-PAGE]
We expressed Dronpa 145N, 145K and luciferase fusion protein at pcold vector in E.coli and obtained these proteins at His-tag purification.