Team:UCLA/Notebook/Protein Cages/1 July 2015
Phillip's notes:
Introduction: Today the project overview will be discussed during our general meeting with Dr. Kosuri. Fasih will then help us take our glycerol stock from Dr. Yeates and plate them to select for making a starter culture tomorrow.
Meetng notes: site direct mutagenesis with whole plasmid. Need DpnI
Try Gibson assembly: Need Gibson and PCR enzymes, DpnI (cuts methylated GATC to remove old plasmid)
Upon retrieving the clones, it was realized that they were in the -20 degree fridge instead of the -80 degree fridge. The entirety of the tube was used to plate the cells on an agar plate with ampicillin, with the help of Tristan. He did not work over a flame.
Conclusions: If colonies grow, we will make a starter culture tomorrow, and the large culture Friday. Hopefully something grows on our plates :(