Team:UCLA/Collaborations
SilkyColi: Reprogramming the physical and functional properties of synthetic silks
Collaborations
Cloning Guide with TU Eindhoven
First, we were very motivated to discuss the potential for iterative capped assembly to open the doors for other iGEM teams to explore new features of protein synthesis. To achieve this, we collaborated with the great members of the TU Eindhoven iGEM Team, for whom we developed a cloning guide for ICA. Our entry in their guide discussing the background, process, workflow, and troubleshooting tips necessary to utilize this system. Our collaboration has not only benefited iGEM teams who wish to learn the technique, but also helped us gain a better sense of the implications of our research toward advanced synthetic biology. We envision ICA as a cloning technique that can be widely adapted not only to those using our parts collection generate novel silk genes, but for any team who wishes to rapidly and controllably assembly long repetitive genetic structures for functional use.
