Team:UCLA/Collaborations

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SilkyColi: Reprogramming the physical and functional properties of synthetic silks

Collaborations

Cloning Guide with TU Eindhoven

First, we were very motivated to discuss the potential for iterative capped assembly to open the doors for other iGEM teams to explore new features of protein synthesis. To achieve this, we collaborated with the great members of the TU Eindhoven iGEM Team, for whom we developed a cloning guide for ICA. Our entry in their guide discussing the background, process, workflow, and troubleshooting tips necessary to utilize this system. Our collaboration has not only benefited iGEM teams who wish to learn the technique, but also helped us gain a better sense of the implications of our research toward advanced synthetic biology. We envision ICA as a cloning technique that can be widely adapted not only to those using our parts collection generate novel silk genes, but for any team who wishes to rapidly and controllably assembly long repetitive genetic structures for functional use.

Figure 1: Example introductory page of the ICA method on the TU Eindhoven Cloning Guide. Guide includes details on method background, protocols and workflow needed to design assembly pieces, and FAQ/troubleshooting guidelines.

First Annual Southern California iGEM Meetup

The UCLA iGEM was honored to hold the first ever Southern California iGEM Meetup on Thursday, August 6th. Team members from the University of California, San Diego (UCSD), University of La Verne and La Cañada High School, and the LA Biohackers Community Lab attend our meetup at the Molecular Biology Institute at UCLA. Here is a link to the powerpoint we delivered at the meet up, and below are pictures from our event. Teams discussed their ongoing projects and offered advice to members struggling with certain aspects of their experimental design, and discussed the pertinent issues for all iGEM teams in terms of logistics, team organization and project design. Additionally, we discussed the state of iGEM and synthetic biology as a rapidly emerging biotechnology field in the United States, especially in the context of developing scientific communications to combat rising negative publicity and misinformation toward the use and safety of genetically modified organisms. Through our meet up, we hope to spur not only a collaborative experience in local teams to work together in solving synthetic biology problems, but also a space where teams can discuss maintenance and sustained success of an iGEM team, especially in dire financial circumstances We hope to increase of presence in the California community, and encourage both Northern Californian and local high school teams to attend our meet up, in an effort to sustain further project collaboration.