Team:Czech Republic/Goals

Goals

Contents

1 Goals of module 1
2 Goals of module 2
3 Goals of module 3

Goals of module 1

Construct a set of reporter promoters for yeast cells
Characterize reporter promoters in all mating types
Design synthetic MATa and MATx strains
Build a synthetic diploid strain with a functional yeast pheromone pathway
Demonstrate the correct functionality of yeast pheromone pathway in synthetic diploids

Goals of module 2

Construct a set of yeast plasmids with different mating pheromones and their receptors and contribute to the Registry with the BioBricks
Verify the correct coupling of the receptors to the yeast pheromone mating pathway
Verify the correct expression and secretion of the different pheromones
Show the orthogonality of the used receptors and pheromones

Goals of module 3

Demonstrate the ability of our receptors to bind chosen markers.