Team:Duke/Attributions

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Attributions

Duke iGEM 2015 is grateful for the support we have received from Duke University’s staff, faculty and donors for continuing to allow us the opportunity to explore synthetic biology and the issues that go into it. We appreciate all the help we’ve received and could not have done it without you. Thank you for all your support and advice.

Faculty (Alphabetically)

• Dr. Chase Beisel (North Carolina State University): Giving insight and project ideas on dCas9 circuiting and decoy binding site design.
• Dr. Nicolas Buchler: For advising us on project options, sponsoring our Bass Connections project and student-taught course, and for allowing Duke iGEM to use Buchler Lab space
• Dr. Charles Gersbach: For advising us on project design and lab technique, sponsoring our Bass Connections project and student-taught course as well as offering insights into the uses of Cas9 protein technologies.
• Dr. Mike Lynch: For advising on early dCas9 circuit design prototyping and idea formation.
• Dr. Lingchong You: For providing insights on antibiotic resistance and providing BBa_K#####, the E Lysis gene.

Funding Support:

• Bass Connections
• The continued support of The Cameron Hayden Lord Foundation.

Lab Support:

• The Biomedical Engineering Teaching Lab staff: For providing dedicated lab space and technical support.
• The Buchler Lab: For use of lab space and equipment as well as wet lab troubleshooting aid. For facilitating the procurement of lab equipment and disposables.
• The Gersbach Lab: For providing troubleshooting aid and advice.

References

"Antibiotic Resistance Threats in the United States, 2013." Centers for Disease Control and Prevention. Centers for Disease Control and Prevention, 17 July 2014. Web. 10 Sept. 2015.

Bernhardt, T. G., D. K. Struck, and R. Young. 'The Lysis Protein E Of Phi X174 Is A Specific Inhibitor Of The Mray-Catalyzed Step In Peptidoglycan Synthesis'. Journal of Biological Chemistry 276.9 (2000): 6093-6097. Web.

Chak, K.-F. et al. 'Cloning And Characterization Of The Cole7 Plasmid'. Journal of General Microbiology 137.1 (1991): 91-100. Web.

Chart, H., H. R. Smith, R. M. La Ragione, and M. J. Woodward. "An Investigation into the Pathogenic Properties of Escherichia Coli Strains BLR, BL21, DH5alpha and EQ1." J Appl Microbiol Journal of Applied Microbiology 89.6 (2000): 1048-058. PubMed. Web.

"Drug-Resistant TB." Centers for Disease Control and Prevention. Centers for Disease Control and Prevention, 01 Apr. 2014. Web. 18 Sept. 2015.

Frecer, Vladimir. 'QSAR Analysis Of Antimicrobial And Haemolytic Effects Of Cyclic Cationic Antimicrobial Peptides Derived From Protegrin-1'. Bioorganic & Medicinal Chemistry 14.17 (2006): 6065-6074. Web.

KATSU, Takashi, Satoshi NAKAO, and Sadaaki IWANAGA. 'Mode Of Action Of An Antimicrobial Peptide, Tachyplesin I, On Biomembranes.'. Biol. Pharm. Bull. 16.2 (1993): 178-181. Web.

Marguet, Philippe et al. 'Oscillations By Minimal Bacterial Suicide Circuits Reveal Hidden Facets Of Host-Circuit Physiology'. PLoS ONE 5.7 (2010): e11909. Web.

Mohanram, H., and S. Bhattacharjya. "Cysteine Deleted Protegrin-1 (CDP-1): Anti-bacterial Activity, Outer-Membrane Disruption and Selectivity." Biochimica Et Biophysica Acta (BBA) - General Subjects 1840.10 (2014): 3006-016. ScienceDirect. Web. 16 June 2015.

Parts.igem.org,. 'Part:Bba K117000 - Parts.Igem.Org'. N.p., 2015. Web. 18 Sept. 2015.

Parts.igem.org,. 'Part:Bba K628000 - Parts.Igem.Org'. N.p., 2015. Web. 18 Sept. 2015.

Qi, Lei S., Matthew H. Larson, Luke A. Gilbert, Jennifer A. Doudna, Jonathan S. Weissman, Adam P. Arkin, and Wendell A. Lim. "Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression." Cell 152.5 (2013): 1173-183. NCBI. Web. 18 Sept. 2015.

Ramamoorthy, Ayyalusamy et al. 'Deletion Of All Cysteines In Tachyplesin I Abolishes Hemolytic Activity And Retains Antimicrobial Activity And Lipopolysaccharide Selective Binding'. Biochemistry 45.20 (2006): 6529-6540. Web.

Shankaramma, Sasalu C. et al. 'Macrocyclic Hairpin Mimetics Of The Cationic Antimicrobial Peptide Protegrin 1: A New Family Of Broad-Spectrum Antibiotics.'. ChemInform 34.4 (2003): n. pag. Web.

Tam, James P., Chengwei Wu, and Jin-Long Yang. 'Membranolytic Selectivity Of Cystine-Stabilized Cyclic Protegrins'. European Journal of Biochemistry 267.11 (2000): 3289-3300. Web.