Team:UCLA/Notebook/Recombinant Expression/1 July 2015

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Inoculation of Tamura Colony

Plates were streaked two weeks ago with transformed E-coli cells containing an alternating sequence of wild type silk and GFP, specifically: N-terminus-GFP-C-terminus. An isolated colony was picked and inoculated in Lysogeny Broth (commonly referred to as Luria broth, an excellent growth medium for bacteria) overnight for 16 hours.

Procedure:

Colony picked with micropipet and inserted into a culture tube containing 10 mL of LB and 10 ul of Ampicillin.