Team:UCLA/Notebook/Recombinant Expression/1 July 2015

• HOME
• TEAM
• PROJECTS
  • Silk Genetics
  • Functionalization
  • Materials Processing
  • Protein Cages
• PARTS
  • Team Parts
  • Basic Parts
  • Composite Parts
  • Part Collection
• NOTEBOOKS
  • Spider Silk Genetics
  • Honeybee Silk
  • Recombinant Silk Functionalization
  • Materials Processing
  • Protein Cages
  • UCLA Measurement Interlab Study
• MEETING NOTES
  • General Meetings
  • Coordinator Meetings
  • Advisor Meetings
• ATTRIBUTIONS
• COLLABORATIONS
• HUMAN PRACTICES
• SAFETY
• MODELING
• MEASUREMENT STUDY
• SOFTWARE
• ENTREPRENEURSHIP

Inoculation of Tamura Colony

Plates were streaked two weeks ago with transformed E-coli cells containing an alternating sequence of wild type silk and GFP, specifically: N-terminus-GFP-C-terminus. An isolated colony was picked and inoculated in Lysogeny Broth (commonly referred to as Luria broth, an excellent growth medium for bacteria) overnight for 16 hours.

Procedure: Colony picked with micropipet and inserted into a culture tube containing 10 mL of LB and 10 ul of Ampicillin.