Team:UCLA/Notebook/Recombinant Expression/6 July 2015
Cell Lysis, continued
Calculations of Reagent Quantities Needed (accounting for inflation of cell pellet weight due to inclusion of Falcon tube mass)
Mass of Cell Pellet | Lysozyme | PMSF Solution | Deoxycholic Acid |
---|---|---|---|
0.52g | 0.32g | 0.42g | |
0.0082g | 0.0082g | 0.0084g | |
0.41mL | 0.41mL | 0.42mL |