Team:UCLA/Notebook/Recombinant Expression/6 July 2015

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Cell Lysis, continued Calculations of Reagent Quantities Needed (accounting for inflation of cell pellet weight due to inclusion of Falcon tube mass)

Mass of Cell Pellet	Lysozyme	PMSF Solution	Deoxycholic Acid
0.52g	0.0082g	0.41mL	0.78g
0.32g	0.0082g	0.41mL	0.48g
0.42g	0.0084g	0.42mL	0.63g
0.52g	0.0084g	0.42mL	0.78g